Expression profiling of IL-1β, IL-6 and IL-8 genes in lung tissues of Aseel, Crossbred Naked neck, and White Leghorn chicken challenged with H9N2 Influenza virus

Aseel and Naked neck are major chicken breeds of the tropics well-known for their thermotolerance robustness. However, both them especially very susceptible to Avian Influenza (AI) which cause huge mortalities. The role cytokines in relation pathology severity disease caused by endemic strain (H9N2) AIV these remained thus far unclear. aim this study was investigate effects H9N2 on expression level IL-1β, IL-6 IL-8 lung tissues Aseel, crossbred Neck, White Leghorn (WLH). In total 60 birds, 20 from each breed, were used study, whereas 30 birds (10 breed) challenged intranasally with virus a concentration 106 EID50 at 6wk age other half treated as control. sampled 5th day post-infection differential IL-6, using qRT-PCR. Our data revealed significant difference (P<0.001) gene levels among all response viral challenge. It also observed that after exposure virus, showed highest increase expressions interleukin (IL-1 β, IL-8) genes followed Neck WLH respectively suggesting greater susceptibility compared breeds. results agreement incidence mortality AI